Regulatory

Part:BBa_K511000:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)

Hef1a-LacOid Promoter MammoBlock


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 292
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 670
    Illegal BamHI site found at 255
    Illegal XhoI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
    Illegal NgoMIV site found at 804
    Illegal AgeI site found at 80
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When sequencing constructs that include this part, it is important to realize that the Hef1a-LacOid promoter is roughly a kilobase long; therefore, promoter-gene pairs will require either internal sequencing primers or sequencing from the gene side of the construct to be useful.


Source

This part is a synthetic promoter formed from the human elongation factor 1a promoter sequence and a LacOid operator that has been computationally shown to bind to the LacI repressor protein more tightly than the wildtype LacO operator. Sincere thanks go to the Weiss lab for contributing this entry vector to the MIT iGEM 2011 team.

References